Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin

Abstract

The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant.

Keywords: 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) (PubChem CID:2723939); 2-(N-morpholino)-ethanesulfonic acid (Pubchem CID:4478249); 4′,6-Diamidino-2-phenylindole (PubChem CID:2954); Bone formation; Bone morphogenetic protein-2 (BMP-2); Dopamine hydrochloride (PubChem CID:65340); Heparin; Magnesium chloride (PubChem CID:24584); N-hydroxysuccinimide (PubChem CID:80170); Osteoblast function; Phenylmethylsulfonyl fluoride (PubChem CID:4784); Sodium azide (PubChem CID:33557); Sodium chloride (PubChem CID:5234); Titanium (Ti); Tris-hydrochloride (PubChem CID:10997301).