Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas

J Dairy Sci. 2014 Dec;97(12):7668-73. doi: 10.3168/jds.2013-7637. Epub 2014 Sep 26.

Abstract

The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.

Keywords: Streptococcus uberis; Streptococcus uberis adhesion molecule; dairy cow; mastitis; sua gene sequencing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacterial Adhesion / physiology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cattle
  • Cell Adhesion Molecules
  • Conserved Sequence
  • Female
  • Gene Expression Regulation, Bacterial / physiology*
  • Genetic Variation
  • Milk
  • Polymerase Chain Reaction
  • Streptococcus / genetics
  • Streptococcus / metabolism*

Substances

  • Bacterial Proteins
  • Cell Adhesion Molecules