Cloning and expression of a human muscle phosphofructokinase cDNA

Gene. 1989 Apr 15;77(1):177-83. doi: 10.1016/0378-1119(89)90372-7.

Abstract

The nucleotide sequence of a 2.86-kb cDNA clone containing the complete human muscle phosphofructokinase (PFK) protein-coding region was determined. It comprises 76 bp of 5'-untranslated sequence, 2340 bp encoding human muscle PFK polypeptide, and 399 bp of 3'-untranslated sequence plus a poly(A) tract. A retroviral vector was utilized to express the product of this coding sequence in mouse fibroblasts. The PFK-coding cDNA was shown to code for an enzymatically active polypeptide by immunoprecipitation analysis and DEAE-Sephadex A-25 chromatography.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromatography, Ion Exchange
  • Cloning, Molecular*
  • DNA / biosynthesis
  • DNA / genetics
  • DNA / isolation & purification
  • Genes*
  • Genetic Vectors
  • Humans
  • Molecular Sequence Data
  • Muscles / enzymology*
  • Phosphofructokinase-1 / biosynthesis
  • Phosphofructokinase-1 / genetics*
  • Phosphofructokinase-1 / metabolism
  • Precipitin Tests
  • Retroviridae / genetics
  • Transfection

Substances

  • DNA
  • Phosphofructokinase-1

Associated data

  • GENBANK/M26066