Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes

C Levrat; D Ardail; R Morelis; P Louisot

doi:10.1016/0020-711x(89)90184-5

Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes

Int J Biochem. 1989;21(3):265-78. doi: 10.1016/0020-711x(89)90184-5.

Authors

C Levrat¹, D Ardail, R Morelis, P Louisot

Affiliation

¹ Department of Biochemistry, University of Lyon, Lyon-Sud Medical School, INSERM-CNRS U.189, Oullins, France.

PMID: 2526034
DOI: 10.1016/0020-711x(89)90184-5

Abstract

1. In the mitochondria, the biosynthesis of N-glycoprotein products, through the dolichol intermediates pathway, appears in the outer and in the inner membranes. 2. The biosynthesis of dolichol-pyrophosphoryl-N-acetyl-glucosamine, dolichol-pyrophosphoryl-di-N-acetylchitobiose, dolichol-phosphoryl-glucose and dolichol-phosphoryl-mannose is effective in both membranes. 3. The lipid-linked oligosaccharides biosynthesized in both membranes contain high mannose-type oligosaccharides ranging in size from Man9-GlcNac2 to Man4-GlcNac2. 4. The assembly of the dolichol-pyrophosphoryl-oligosaccharides on the trimannosidic core begins by the elongation of the alpha-1,3 mannose branch in the outer membrane and of the alpha-1,6 mannose branch in the inner membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1-Deoxynojirimycin
Acetylglucosaminidase
Animals
Cell Fractionation
Chromatography, Liquid / methods
Dolichols / metabolism*
Glucosamine / analogs & derivatives
Glucosamine / pharmacology
Glycoproteins / biosynthesis*
In Vitro Techniques
Intracellular Membranes / metabolism
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Mice
Mitochondria / metabolism*

Substances

Dolichols
Glycoproteins
1-Deoxynojirimycin
Acetylglucosaminidase
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Glucosamine