Maintenance of leptospira species in leptospira Vanaporn Wuthiekanun agar

Vanaporn Wuthiekanun; Premjit Amornchai; Sayan Langla; Malinee Oyuchua; Nicholas P J Day; Direk Limmathurotsakul

doi:10.1128/JCM.02273-14

Maintenance of leptospira species in leptospira Vanaporn Wuthiekanun agar

J Clin Microbiol. 2014 Dec;52(12):4350-2. doi: 10.1128/JCM.02273-14. Epub 2014 Sep 24.

Authors

Vanaporn Wuthiekanun¹, Premjit Amornchai¹, Sayan Langla¹, Malinee Oyuchua¹, Nicholas P J Day², Direk Limmathurotsakul³

Affiliations

¹ Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
² Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Nuffield Department of Medicine, University of Oxford, Old Road Campus, Headington, Oxford, United Kingdom.
³ Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand direk@tropmedres.ac.

Abstract

The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar
Bacteriological Techniques / methods*
Culture Media / chemistry*
Humans
Leptospira / physiology*
Microbial Viability
Preservation, Biological / methods*
Time Factors

Substances

Culture Media
Agar

Grants and funding

089275/Z/09/Z/Wellcome Trust/United Kingdom