Aptamer-functionalized barcode particles for the capture and detection of multiple types of circulating tumor cells

Adv Mater. 2014 Nov 19;26(43):7333-8. doi: 10.1002/adma.201403530. Epub 2014 Sep 23.

Abstract

Aptamer-functionalized barcode particles are employed to capture and detect various types of circulating tumor cells (CTCs). The particles are spherical colloidal crystal clusters, and the reflection properties that arise from their structures are how their codes are evaluated. Aptamer functionalization (with TD05, Sgc8, and Sgd5) make the particles interact with specific CTC types; dendrimers are used to amplify the effect of the aptamers, allowing for increased sensitivity, reliability, and specificity in CTC capture, detection, and subsequent release.

Keywords: aptamers; biomedical applications; colloidal crystals; dendrimers; tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Cell Line, Tumor
  • Cell Separation / methods*
  • Dendrimers / chemistry
  • Humans
  • Leukemia / diagnosis
  • Leukocyte Common Antigens / chemistry
  • Leukocytes / chemistry
  • Lymphoma / chemistry
  • Lymphoma / diagnosis
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Nanoparticles / chemistry
  • Neoplastic Cells, Circulating / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Silicon Dioxide / chemistry

Substances

  • Aptamers, Nucleotide
  • Dendrimers
  • Silicon Dioxide
  • Leukocyte Common Antigens
  • PTPRC protein, human