Label-free and non-invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum-free media

Abstract

Traditional approaches to characterize stem cell differentiation are time-consuming, lengthy and invasive. Here, Raman microspectroscopy (RM) and atomic force microscopy (AFM) - both considered as non-invasive techniques - are applied to detect the biochemical and biophysical properties of trophoblast derived stem-like cells incubated up to 10 days under conditions designed to induce differentiation. Significant biochemical and biophysical differences between control cells and differentiated cells were observed. Quantitative real time PCR was also applied to analyze gene expression. The relationship between cell differentiation and associated cellular biochemical and biomechanical changes were discussed. Monitoring trophoblast cells differentiation.

Keywords: Raman microspectroscopy; atomic force microscopy (AFM); cellular differentiation; principal component analysis (PCA); qPCR; transfected porcine trophoblast derived cells.