The study of enzymatic and protective properties of recombinant IgA1 protease in active and mutant form showed that active form of IgA1 protease exhibited species - and type-specificity for mouse and human immunoglobulins. Mutant form, which did not exhibit enzymatic activity, had protective properties against meningococcal infection, induced by meningococcus serogroup A, B and C protecting the mice from lethal infection by living virulent culture of heterologous serogroups of meningococcus. Obtained results make it possible to consider IgA1 protease as a perspective preparation at the stages of development of polyvalent vaccine for protection the people from meningococcal infection of various etiology.
NIssledovanie fermentativnykh i protektivnykh svoĭstv rekombinantnoĭ IgA1 proteazy v aktivnoĭ i mutantnoĭ formakh pokazalo, chto aktivnaia forma IgA1 proteazy kharakterizuetsia vidovoĭ i tipovoĭ spetsifichnost'iu v otnoshenii immunoglobulinov myshi i cheloveka. Mutantnaia forma, ne obladaia fermentativnoĭ aktivnost'iu, proiavliala protektivnye svoĭstva v otnoshenii meningokokkovoĭ infektsii, vyzyvaemoĭ meningokokkami serogrupp A, V i S, zashchishchaia mysheĭ ot letal'nogo zarazheniia zhivoĭ virulentnoĭ kul'turoĭ geterologichnykh serogrupp meningokokka. Poluchennye rezul'taty pozvoliaiut rassmatrivat' IgA1 proteazu v kachestve perspektivnogo preparata pri sozdanii polivalentnoĭ vaktsiny dlia zashchity cheloveka ot meningokokkovoĭ infektsii razlichnoĭ étiologii.
Keywords: IgA1 protease; enzymatic activity; immunogenicity; meningococcal vaccine; protective properties.