Complete genome sequence of Peptoniphilus sp. strain ING2-D1G isolated from a mesophilic lab-scale completely stirred tank reactor utilizing maize silage in co-digestion with pig and cattle manure for biomethanation

J Biotechnol. 2014 Dec 20:192 Pt A:59-61. doi: 10.1016/j.jbiotec.2014.09.011. Epub 2014 Sep 19.

Abstract

The bacterium Peptoniphilus sp. strain ING2-D1G (DSM 28672), a mesophilic and obligate anaerobic bacterium belonging to the order Clostridiales was isolated from a biogas-producing lab-scale completely stirred tank reactor (CSTR) optimized for anaerobic digestion of maize silage in co-fermentation with pig and cattle manure. In this study, the whole genome sequence of Peptoniphilus sp. strain ING2-D1G, a new isolate potentially involved in protein breakdown and acidogenesis during biomass degradation, is reported. The chromosome of this strain is 1.6Mb in size and encodes genes predicted to be involved in the production of acetate, lactate and butyrate specifying the acidogenic metabolism of the isolate.

Keywords: Anaerobic digestion; Biomass degradation; Biomethanation; Clostridiales; Protein degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / genetics*
  • Bacteria / metabolism
  • Base Sequence
  • Biofuels
  • Bioreactors
  • Cattle
  • Genome, Bacterial*
  • Manure
  • Methane / biosynthesis
  • Molecular Sequence Data
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Swine
  • Zea mays

Substances

  • Biofuels
  • Manure
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Methane