The Grey nightjar Caprimulgus jotaka is a species of migratory nightjar found in East Asia. In this study, we determined its complete mtDNA by PCR-based method. The mitochondrial genome of Caprimulgus jotaka is a 17,536 bp circular molecule with the A + T content of 53.82%, which contains 37 typical mitochondrial genes (13 protein-coding genes, 2 rRNAs and 22 tRNAs) and a non-coding control region (D-loop). All protein-coding genes are initiated by ATG codon, except for the ND1 gene starts with GTG codon, and ND3 uses ATA codon. TAA is the most frequent stop codon. All tRNAs possess the classic cloverleaf secondary structure except for tRNA(Ser(AGY)) and tRNA(Leu(CUN)), which lacks the ''DHU'' stem. The D-loop is a 1969 bp long AT-rich region, which located between tRNA(Glu) and tRNA(Phe).
Keywords: Caprimulgus jotaka; Grey nightjar; complete mitogenome; gene arrangement.