Abstract
Metabolic syndrome (MetS) induces serious complications; therefore, we developed a noninvasive MetS model using an extremely small minipig, the Microminipig. For 8 weeks, Microminipigs were administrated a high-fat and high-cholesterol diet (HFCD) for atherosclerosis and N(G)-nitro-l-arginine methyl ester (l-NAME) for inhibiting nitric oxide synthase. HFCD significantly increased serum low-density lipoprotein levels, l-NAME increased blood pressure and cardiac hypertrophy, and HFCD-induced aortal arteriosclerosis was accelerated by l-NAME administration. Endothelium-dependent relaxation of the coronary artery was remarkably decreased by l-NAME administration. This model may be useful for elucidating the mechanisms of MetS and developing new therapeutic medicines for its treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Atherosclerosis / etiology
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Blood Pressure / drug effects
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Cardiomegaly / chemically induced
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Cholesterol, Dietary / administration & dosage*
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Cholesterol, Dietary / adverse effects
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Coronary Vessels / drug effects
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Diet, High-Fat* / adverse effects
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Disease Models, Animal*
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Drug Discovery
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Endothelium, Vascular / physiology
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Lipoproteins, LDL / blood
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Male
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Metabolic Syndrome* / drug therapy
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Metabolic Syndrome* / etiology
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Metabolic Syndrome* / physiopathology
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Muscle Relaxation / drug effects
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Muscle, Smooth, Vascular / drug effects
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NG-Nitroarginine Methyl Ester / administration & dosage
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NG-Nitroarginine Methyl Ester / adverse effects
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Nitric Oxide Synthase / antagonists & inhibitors
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Swine
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Swine, Miniature*
Substances
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Cholesterol, Dietary
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Lipoproteins, LDL
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Nitric Oxide Synthase
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NG-Nitroarginine Methyl Ester