Structure and gene cluster organization of the O-antigen of Providencia alcalifaciens O45:H25

Olga G Ovchinnikova; Alexander S Shashkov; Magdalena Moryl; Bin Liu; Antoni Rozalski; Yuriy A Knirel

doi:10.1016/j.carres.2014.07.008

Structure and gene cluster organization of the O-antigen of Providencia alcalifaciens O45:H25

Carbohydr Res. 2014 Oct 29:398:72-6. doi: 10.1016/j.carres.2014.07.008. Epub 2014 Jul 21.

Authors

Olga G Ovchinnikova¹, Alexander S Shashkov¹, Magdalena Moryl², Bin Liu³, Antoni Rozalski², Yuriy A Knirel⁴

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
² Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland.
³ TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, PR China.
⁴ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: yknirel@gmail.com.

PMID: 25240185
DOI: 10.1016/j.carres.2014.07.008

Abstract

O-Polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O45:H25 and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeat of the O-polysaccharide was established: [structure: see text]. The O-antigen gene cluster of P. alcalifaciens O45 was sequenced and found to be in full agreement with the O-polysaccharide structure established.

Keywords: Bacterial polysaccharide structure; Lipopolysaccharide; O-Antigen gene cluster; Providencia alcalifaciens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Molecular Sequence Data
Multigene Family*
O Antigens / chemistry*
O Antigens / genetics*
Providencia / chemistry*
Providencia / genetics*

Substances

O Antigens