A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7(T), isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus Gryllotalpicola, showing more than 97.0 % sequence similarity with Gryllotalpicola daejeonensis RU-04(T) (98.0 %), Gryllotalpicola koreensis RU-16(T) (97.7 %) and Gryllotalpicola kribbensis PU-02(T) (97.3 %). However, DNA-DNA relatedness values demonstrated that strain KIS12-7(T) could be clearly distinguished from closely related species of the genus Gryllotalpicola. The cell-wall peptidoglycan of strain KIS12-7(T) was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7(T) is a representative of a novel species of the genus Gryllotalpicola, and the name Gryllotalpicola soli sp. nov. is proposed; the type strain is KIS12-7(T) ( = DSM 27182(T) = KACC 17302(T) = NBRC 109659(T)).
© 2014 IUMS.