Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling

Yoshikazu Kumashiro; Jun Ishihara; Terumasa Umemoto; Kazuyoshi Itoga; Jun Kobayashi; Tatsuya Shimizu; Masayuki Yamato; Teruo Okano

doi:10.1002/smll.201400787

Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling

Small. 2015 Feb 11;11(6):681-7. doi: 10.1002/smll.201400787. Epub 2014 Sep 19.

Authors

Yoshikazu Kumashiro¹, Jun Ishihara, Terumasa Umemoto, Kazuyoshi Itoga, Jun Kobayashi, Tatsuya Shimizu, Masayuki Yamato, Teruo Okano

Affiliation

¹ Institute of Advanced Biomedical Engineering and Science (TWIns), Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.

PMID: 25238273
DOI: 10.1002/smll.201400787

Abstract

A stripe-patterned thermo-responsive surface is prepared to enable cell separation without labeling. The thermo-responsive surface containing a 3 μm striped pattern exhibits various cell adhesion and detachment properties. A mixture of three cell types is separated on the patterned surface based on their distinct cell-adhesion properties, and the composition of the cells is analyzed by flow cytometry.

Keywords: biomaterials; cell adhesion; cell cultures; cell separation; label-free; patterning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemical synthesis
Acrylic Resins / chemistry
Animals
Cell Culture Techniques / instrumentation*
Cell Culture Techniques / methods
Cell Separation / instrumentation*
Cell Separation / methods
Cells, Cultured
Coated Materials, Biocompatible / chemistry
HeLa Cells
Human Umbilical Vein Endothelial Cells
Humans
Materials Testing
Mice
NIH 3T3 Cells
Staining and Labeling / instrumentation
Staining and Labeling / methods
Surface Properties
Temperature*
Tissue Engineering / instrumentation
Tissue Engineering / methods
Tissue Scaffolds / chemistry*

Substances

Acrylic Resins
Coated Materials, Biocompatible
poly-N-isopropylacrylamide