Stable SET knockdown in breast cell carcinoma inhibits cell migration and invasion

Biochem Biophys Res Commun. 2014 Oct 10;453(1):7-12. doi: 10.1016/j.bbrc.2014.09.013. Epub 2014 Sep 16.

Abstract

Breast cancer is the most malignant tumor for women, however, the mechanisms underlying this devastating disease remain unclear. SET is an endogenous inhibitor of protein phosphatase 2A (PP2A) and involved in many physiological and pathological processes. SET could promote the occurrence of tumor through inhibiting PP2A. In this study, we explore the role of SET in the migration and invasion of breast cancer cells MDA-MB-231 and ZR-75-30. The stable suppression of SET expression through lentivirus-mediated RNA interference (RNAi) was shown to inhibit the growth, migration and invasion of breast cancer cells. Knockdown of SET increases the activity and expression of PP2Ac and decrease the expression of matrix metalloproteinase 9 (MMP-9). These data demonstrate that SET may be involved in the pathogenic processes of breast cancer, indicating that SET can serve as a potential therapeutic target for the treatment of breast cancer.

Keywords: Breast cancer; MMP-9; PP2Ac; RNAi; SET.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / pathology*
  • Breast Neoplasms / physiopathology*
  • Breast Neoplasms / therapy
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Cell Proliferation / physiology
  • DNA-Binding Proteins
  • Female
  • Gene Knockdown Techniques
  • Histone Chaperones / antagonists & inhibitors*
  • Histone Chaperones / genetics
  • Histone Chaperones / physiology
  • Humans
  • Matrix Metalloproteinase 9 / metabolism
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / physiopathology
  • Neoplasm Invasiveness / prevention & control
  • Protein Phosphatase 2 / antagonists & inhibitors
  • Protein Phosphatase 2 / metabolism
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Transcription Factors / antagonists & inhibitors*
  • Transcription Factors / genetics
  • Transcription Factors / physiology

Substances

  • DNA-Binding Proteins
  • Histone Chaperones
  • RNA, Small Interfering
  • SET protein, human
  • Transcription Factors
  • Protein Phosphatase 2
  • Matrix Metalloproteinase 9
  • Mmp9 protein, mouse