Abstract
A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques / methods*
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Cross Infection / epidemiology
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Cross Infection / microbiology
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Gram-Negative Bacteria / classification*
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Gram-Negative Bacteria / genetics
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Gram-Negative Bacteria / isolation & purification
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Gram-Negative Bacterial Infections / epidemiology
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Gram-Negative Bacterial Infections / microbiology*
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Gram-Positive Bacteria / classification*
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Gram-Positive Bacteria / genetics
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Gram-Positive Bacteria / isolation & purification
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Gram-Positive Bacterial Infections / epidemiology
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Gram-Positive Bacterial Infections / microbiology*
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Humans
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Molecular Epidemiology / methods*
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Real-Time Polymerase Chain Reaction / methods*
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Time Factors
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Transition Temperature