Study of isobaric grape seed proanthocyanidins by MALDI-TOF MS

Fabiola De Marchi; Roberta Seraglia; Laura Molin; Pietro Traldi; Antonio Dalla Vedova; Massimo Gardiman; Mirko De Rosso; Riccardo Flamini

doi:10.1002/jms.3422

Study of isobaric grape seed proanthocyanidins by MALDI-TOF MS

J Mass Spectrom. 2014 Sep;49(9):826-30. doi: 10.1002/jms.3422.

Authors

Fabiola De Marchi¹, Roberta Seraglia, Laura Molin, Pietro Traldi, Antonio Dalla Vedova, Massimo Gardiman, Mirko De Rosso, Riccardo Flamini

Affiliation

¹ CRA-VIT, Viale XXVIII aprile 26, 31015, Conegliano, Treviso, Italy.

PMID: 25230179
DOI: 10.1002/jms.3422

Abstract

Positive matrix-assisted laser desorption ionization (MALDI) spectra of grape seed raw extracts show the signals of putative proanthocyanidin (PA) oligomers, including those of several sodium and potassium adducts with the same nominal molecular weight. As a result, the MALDI time-of-flight profiles are characterized by several isobaric signals from different PAs. The presence of isobaric PA adducts was studied by sodium and potassium exchange experiments on the seed extracts of six grape varieties. This approach was effective in differentiating PAs with isobaric signals, and 15 different PAs were identified in the MALDI spectra of all samples.

Keywords: MS; grape seeds, MALDI-TOF; isobaric; proanthocyanidins.