Expression of recombinant proteins by BEVS in a macula-like virus-free silkworm cell line

J Invertebr Pathol. 2014 Nov:123:34-7. doi: 10.1016/j.jip.2014.09.004. Epub 2014 Sep 16.

Abstract

We previously established the first Bombyx mori macula-like virus (BmMLV)-free cell line (BmVF cells) from a B. mori embryo. In this study, we evaluated the expression of recombinant proteins in BmVF cells using a B. mori nucleopolyhedrovirus (BmNPV)-derived expression vector. Our results showed that BmVF cells are susceptible to BmNPV, and both the promoter activity of the polyhedrin gene and the post-translated modifications of a recombinant protein are equivalent between BmMLV-negative BmVF and -positive BmN4 cells. These findings indicate that persistent infection with BmMLV has no discernible effect on BmNPV-mediated protein production in B. mori cells.

Keywords: BEVS; Baculovirus; BmMLV; BmNPV; Maculavirus; Nucleopolyhedrovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Bombyx / virology*
  • Cell Line
  • Nucleopolyhedroviruses*
  • Recombinant Proteins / biosynthesis
  • Tymoviridae
  • Virology / methods*

Substances

  • Recombinant Proteins