Glucose biosensing using glassy carbon electrode modified with polyhydroxy-C60, glucose oxidase and ionic-liquid

Biomed Mater Eng. 2014;24(6):2197-202. doi: 10.3233/BME-141031.

Abstract

Direct electrochemistry of glucose oxidase (GOD) was achieved when an ionic liquid/GOD-Polyhydroxy-C60 functional membrane was confined on a glassy carbon electrode (GCE). The cyclic voltammograms (CVs) of the modified GCE showed a pair of redox peaks with a formal potential (E°') of - 329 ± 2 mV. The heterogeneous electron transfer constant (k(s)) was 1.43 s-1. The modified GCE response to glucose was linear in the range from 0.02 to 2.0 mM. The detection limit was 1 μM. The apparent Michaelis-Menten constant (K(m)(app)) was 1.45 mM.

Keywords: Glucose oxidase; direct electrochemistry; ionic liquid; polyhydroxy-C60.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Carbon / chemistry
  • Carboxylic Acids / chemistry*
  • Coated Materials, Biocompatible / chemistry
  • Conductometry / instrumentation*
  • Electrodes*
  • Equipment Design
  • Equipment Failure Analysis
  • Fullerenes / chemistry*
  • Glass / chemistry
  • Glucose / analysis*
  • Glucose / chemistry
  • Glucose Oxidase / chemistry*
  • Ionic Liquids / chemistry*

Substances

  • C60 carboxyfullerene
  • Carboxylic Acids
  • Coated Materials, Biocompatible
  • Fullerenes
  • Ionic Liquids
  • Carbon
  • Glucose Oxidase
  • Glucose