In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.
Keywords: MDM2; cell imaging; p53; protein-protein interaction; small-molecule fluorescent probe.
© 2014 John Wiley & Sons A/S.