A fluorescent probe for imaging p53-MDM2 protein-protein interaction

Chem Biol Drug Des. 2015 Apr;85(4):411-7. doi: 10.1111/cbdd.12434. Epub 2014 Oct 10.

Abstract

In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.

Keywords: MDM2; cell imaging; p53; protein-protein interaction; small-molecule fluorescent probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Humans
  • Optical Imaging / methods*
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Protein Interaction Maps*
  • Proto-Oncogene Proteins c-mdm2 / metabolism*
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Fluorescent Dyes
  • Tumor Suppressor Protein p53
  • Proto-Oncogene Proteins c-mdm2