Lacidipine attenuates TNF-α-induced cardiomyocyte apoptosis

Cytokine. 2015 Jan;71(1):60-5. doi: 10.1016/j.cyto.2014.08.004. Epub 2014 Sep 16.

Abstract

This study was designed to investigate whether lacidipine elicited a protective role on cardiomyocyte against apoptosis induced by TNF-α. Neonatal rat cardiomyocytes were randomly assigned into different groups. TUNEL staining was utilized to detect apoptosis, and caspase-3 and caspse-12 were determined. To explore the underlying mechanism, Z-ATAD-FMK (a selective caspase-12 inhibitor) was used to identify the key molecule involved. TNF-α increased caspase-3 expression, which was mediated by increased caspase-12 expression. In the meantime, apoptosis was significantly induced by TNF-α. Lacidipine lowered caspase-12 and caspase-3 expression, and cardiomyocyte apoptosis induced by TNF-α. The results suggest that lacidipine attenuates TNF-α -induced apoptosis via inhibition of caspase-12 and caspase-3 successively.

Keywords: Apoptosis; Caspase-12; Lacidipine; TNF-α.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Calcium Channel Blockers / pharmacology*
  • Caspase 12 / genetics
  • Caspase 12 / metabolism
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Cells, Cultured
  • Dihydropyridines / pharmacology*
  • In Situ Nick-End Labeling
  • Male
  • Myocytes, Cardiac / drug effects*
  • Myocytes, Cardiac / physiology
  • Random Allocation
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Calcium Channel Blockers
  • Dihydropyridines
  • Tumor Necrosis Factor-alpha
  • lacidipine
  • Caspase 12
  • Caspase 3