N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Anal Bioanal Chem. 2014 Nov;406(28):7049-57. doi: 10.1007/s00216-014-8119-7. Epub 2014 Sep 12.

Abstract

Characterization and optimization studies of N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H2O2 concentration, metal-cation type, and the concentration and type of surfactant on the fluorescence intensity were investigated. The optimized reaction was subsequently used for the development of an assay for DNA detection based on a molecular-beacon probe. The use of a fluorogenic substrate enabled the detection of a single-stranded DNA target with a 1 nmol L(-1) detection limit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • DNA, Catalytic / metabolism*
  • DNA, Single-Stranded / analysis*
  • DNA, Single-Stranded / chemistry
  • Fluorescent Dyes / chemistry*
  • Hydrazines / metabolism*
  • Hydrogen Peroxide
  • Limit of Detection
  • Oxadiazoles / metabolism*
  • Peroxidases / metabolism*

Substances

  • DNA, Catalytic
  • DNA, Single-Stranded
  • Fluorescent Dyes
  • Hydrazines
  • N-methyl-4-hydrazino-7-nitrobenzofurazan
  • Oxadiazoles
  • Hydrogen Peroxide
  • Peroxidases