Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

Hong Jiang; Pengfei Lv; Jichang Li; Hongjun Wang; Tiezhong Zhou; Yingzi Liu; Wei Lin

doi:10.3969/j.issn.1673-5374.2013.28.001

Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

Neural Regen Res. 2013 Oct 5;8(28):2597-604. doi: 10.3969/j.issn.1673-5374.2013.28.001.

Authors

Hong Jiang¹, Pengfei Lv², Jichang Li³, Hongjun Wang⁴, Tiezhong Zhou⁴, Yingzi Liu⁴, Wei Lin³

Affiliations

¹ Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China ; Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
² Scientific Research Department, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
³ Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China.
⁴ Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.

PMID: 25206570
PMCID: PMC4146026
DOI: 10.3969/j.issn.1673-5374.2013.28.001

Abstract

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress co-listin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5-500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhi-bited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.

Keywords: PC12 cells; apoptosis; baicalin; caspase-3; colistin sulfate; grants-supported paper; lactate dehydrogenase; neural regeneration; neuroregeneration; traditional Chinese medicine.