Poxue Huayu and Tianjing Busui Decoction for cerebral hemorrhage (Upregulation of neurotrophic factor expression): Upregulation of neurotrophic factor expression

Jixiang Ren; Xiangyu Zhou; Jian Wang; Jianjun Zhao; Pengguo Zhang

doi:10.3969/j.issn.1673-5374.2013.22.003

Poxue Huayu and Tianjing Busui Decoction for cerebral hemorrhage (Upregulation of neurotrophic factor expression): Upregulation of neurotrophic factor expression

Neural Regen Res. 2013 Aug 5;8(22):2039-49. doi: 10.3969/j.issn.1673-5374.2013.22.003.

Authors

Jixiang Ren¹, Xiangyu Zhou², Jian Wang¹, Jianjun Zhao¹, Pengguo Zhang³

Affiliations

¹ Department of Encephalopathy, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, Jilin Province, China.
² Grade 2010 Clinical Medicine Major, School of Clinical Medicine, Yanbian University, Yanji 133002, Jilin Province, China.
³ Department of Imaging, School of Second Clinical Medicine, Jilin University, Changchun 130041, Jilin Province, China.

PMID: 25206512
PMCID: PMC4146063
DOI: 10.3969/j.issn.1673-5374.2013.22.003

Abstract

This study established a rat model of cerebral hemorrhage by injecting autologous anticoagulated blood. Rat models were intragastrically administered 5, 10, 20 g/kg Poxue Huayu and Tianjing Busui Decoction, supplemented with Hirudo, raw rhubarb, raw Pollen Typhae, gadfly, Fructrs Trichosanthis, Radix Notoginseng, Rhizoma Acori Talarinowii, and glue of tortoise plastron, once a day, for 14 consecutive days. Results demonstrated that brain water content significantly reduced in rats with cerebral hemorrhage, and intracerebral hematoma volume markedly reduced after treatment. Immunohistochemical staining revealed that brain-derived neurotrophic factor, tyrosine kinase B and vascular endothelial growth factor expression noticeably increased around the surrounding hematoma. Reverse transcription-PCR revealed that brain-derived neurotrophic factor and tyrosine kinase B mRNA expression significantly increased around the surrounding hematoma. Neurologic impairment obviously reduced. These results indicated that Poxue Huayu and Tianjing Busui Decoction exert therapeutic effects on cerebral hemorrhage by upregulating the expression of brain-derived neurotrophic factor.

Keywords: Poxue Huayu and Tianjing Busui; brain-derived neurotrophic factor; cerebral hemorrhage; grants-supported paper; neural regeneration; neuroregeneration; traditional Chinese medicine; tyrosine kinase B; vascular endothelial growth factor.