Low-level expression of purine bases in BALB/3T3 cells monitored by ultrasensitive graphene-based glass carbon electrode

Anal Biochem. 2014 Dec 15:467:40-6. doi: 10.1016/j.ab.2014.08.034. Epub 2014 Sep 6.

Abstract

Using an ultrasensitive chemically reduced graphene oxide and ionic liquid modified glass carbon electrode (RGI-GCE), separated electrochemical signals of adenine and hypoxanthine in both human breast cancer (MCF-7) and mouse embryonic fibroblast (BALB/3T3) cells were observed. For the first time, low-level expression of purine bases in noncancerous BALB/3T3 cells can be electrochemically monitored. The metabolism of purine bases in carcinogen agent-contaminated BALB/3T3 cells was also investigated through the change of electrochemical signals ascribed to different purine bases, which opens a new electrochemical approach to the exploration of a low-level purine mechanism in noncancerous cells.

Keywords: Cells; Electrochemical; Graphene; Ionic liquid; Purine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analysis*
  • Adenine / metabolism
  • Animals
  • BALB 3T3 Cells
  • Biosensing Techniques / methods
  • Carbon / chemistry*
  • Electrochemical Techniques / methods*
  • Electrodes*
  • Glass / chemistry*
  • Graphite / chemistry*
  • Humans
  • Hypoxanthine / analysis*
  • Hypoxanthine / metabolism
  • MCF-7 Cells
  • Mice
  • Oxidation-Reduction

Substances

  • Hypoxanthine
  • Carbon
  • Graphite
  • Adenine