Revelation of staphylococcal cassette chromosome mec types in methicillin-resistant Staphylococcus aureus isolates from Thailand and Vietnam

R Lawung; L V Chuong; R Cherdtrakulkiat; A Srisarin; V Prachayasittikul

doi:10.1016/j.mimet.2014.08.024

Revelation of staphylococcal cassette chromosome mec types in methicillin-resistant Staphylococcus aureus isolates from Thailand and Vietnam

J Microbiol Methods. 2014 Dec:107:8-12. doi: 10.1016/j.mimet.2014.08.024. Epub 2014 Sep 6.

Authors

R Lawung¹, L V Chuong², R Cherdtrakulkiat³, A Srisarin⁴, V Prachayasittikul⁵

Affiliations

¹ Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Center of Medical Laboratory Services, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
² Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
³ Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
⁴ Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
⁵ Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. Electronic address: virapong.pra@mahidol.ac.th.

PMID: 25205542
DOI: 10.1016/j.mimet.2014.08.024

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is highly prevalent, and its typing plays a crucial role in epidemiology and evolution in both health and community settings. Multiplex PCR and staphylococcal cassette chromosome mec (SCCmec) typing based on mec complexes and cassette chromosome recombinase (ccr) allotypes have been developed for MRSA identification. The first of these procedures can identify 4 mec classes (A, B, C1, and E) and 2 ccr allotypes (B2 and B4) in one tube, and the second can identify mecA, mec class C2, and 3 allotypes (A1, A3, and C). Our method offers a novel means to further differentiate between the main SCCmec types I through XI and is both highly sensitive (detectable up to 0.3ηg DNA) and specific (100%). Several SCCmec types (I, III, IV, V and a non-typeable group) were found in 66 MRSA isolates obtained from Ho Chi Minh City, Vietnam and Nakhon Pathom, Thailand. SCCmec type III was highly predominant in both regions. The designed assay is rapid, convenient, flexible, and reliable. Therefore, this assay is suitable for the high-throughput screening of the main SCCmec types of MRSA isolates.

Keywords: Methicillin resistant Staphylococcus aureus; SCCmec; ccr allotype; mec gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Humans
Methicillin Resistance / genetics*
Methicillin-Resistant Staphylococcus aureus / classification*
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics*
Methicillin-Resistant Staphylococcus aureus / isolation & purification
Microbial Sensitivity Tests
Multilocus Sequence Typing
Multiplex Polymerase Chain Reaction / methods*
Multiplex Polymerase Chain Reaction / standards
Reproducibility of Results
Sensitivity and Specificity
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology*
Thailand
Vietnam

Substances

Anti-Bacterial Agents
Bacterial Proteins