Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)

Haruko Ando; Naoko Emura; Tetsuro Denda; Naoyuki Nakahama; Miho Inoue-Murayama; Yuji Isagi

doi:10.3732/apps.1300094

Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae)

Appl Plant Sci. 2014 Apr 18;2(5):apps.1300094. doi: 10.3732/apps.1300094. eCollection 2014 May.

Authors

Haruko Ando¹, Naoko Emura², Tetsuro Denda³, Naoyuki Nakahama¹, Miho Inoue-Murayama⁴, Yuji Isagi¹

Affiliations

¹ Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
² Department of Life Science, Rikkyo University, Tokyo 171-8501, Japan.
³ Faculty of Science, University of the Ryukyus, Okinawa 903-0213, Japan.
⁴ Wildlife Research Center, Kyoto University, Kyoto 606-8203, Japan.

Abstract

Premise of the study: Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. •

Methods and results: Thirteen microsatellite primer sets were developed for S. taccada using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. •

Conclusions: These results indicate the utility of markers in S. taccada for broad estimations of the population genetic structure of this species.

Keywords: 454 sequencing; Goodeniaceae; Scaevola taccada; genetic structure; seed dispersal.