Isolation of 16 microsatellite markers for Spiraea alpina and S. mongolica (Rosaceae) of the Qinghai-Tibet Plateau

Gulzar Khan; Faqi Zhang; Qingbo Gao; Xiujie Jiao; Pengcheng Fu; Rui Xing; Jinhua Zhang; Shilong Chen

doi:10.3732/apps.1300059

Isolation of 16 microsatellite markers for Spiraea alpina and S. mongolica (Rosaceae) of the Qinghai-Tibet Plateau

Appl Plant Sci. 2014 Jan 7;2(1):apps.1300059. doi: 10.3732/apps.1300059. eCollection 2014 Jan.

Authors

Gulzar Khan¹, Faqi Zhang², Qingbo Gao³, Xiujie Jiao³, Pengcheng Fu³, Rui Xing³, Jinhua Zhang³, Shilong Chen³

Affiliations

¹ Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China ; University of the Chinese Academy of Sciences, Beijing 100039, People's Republic of China.
² Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China ; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, People's Republic of China.
³ Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.

Abstract

Premise of the study: A set of microsatellite markers were developed to characterize the level of genetic diversity and gene flow in two plant species endemic to the Qinghai-Tibet Plateau, Spiraea alpina and S. mongolica. •

Methods and results: Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method, 16 microsatellite loci showed polymorphisms in both species. In two populations of each species, the number of alleles per locus ranged from three to 18 in S. alpina and from four to 30 in S. mongolica. •

Conclusions: These microsatellite markers provide an efficient tool for population genetic studies and will be used to assess the genetic diversity and spatial genetic structure of S. alpina and S. mongolica.

Keywords: Qinghai–Tibet Plateau; Spiraea; gene flow; genetic diversity; microsatellite markers; population genetics.