Isolation of microsatellite markers for the red mangrove, Rhizophora mangle (Rhizophoraceae)

Diana O Ribeiro; Christina C Vinson; Dulcivania S S Nascimento; Ulf Mehlig; Moirah P M Menezes; Iracilda Sampaio; Marivana B Silva

doi:10.3732/apps.1300003

Isolation of microsatellite markers for the red mangrove, Rhizophora mangle (Rhizophoraceae)

Appl Plant Sci. 2013 Sep 2;1(9):apps.1300003. doi: 10.3732/apps.1300003. eCollection 2013 Sep.

Authors

Diana O Ribeiro¹, Christina C Vinson², Dulcivania S S Nascimento¹, Ulf Mehlig¹, Moirah P M Menezes¹, Iracilda Sampaio¹, Marivana B Silva¹

Affiliations

¹ Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
² Molecular Biology and Biochemistry, Universidade Federal de Viçosa, Viçosa, 36570-000 Minas Gerais, Brazil.

Abstract

Premise of the study: Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii. The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species. •

Methods and results: Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii. •

Conclusions: The microsatellite markers presented here will be useful in studies of contemporary and historical gene flow between American and West African Rhizophora species.

Keywords: Rhizophora harrisonii; Rhizophora racemosa; Rhizophoraceae; mangrove; microsatellites.