Co(II)/Co(I) reduction-induced axial histidine-flipping in myoglobin reconstituted with a cobalt tetradehydrocorrin as a methionine synthase model

Takashi Hayashi; Yoshitsugu Morita; Eiichi Mizohata; Koji Oohora; Jun Ohbayashi; Tsuyoshi Inoue; Yoshio Hisaeda

doi:10.1039/c4cc05448b

Co(II)/Co(I) reduction-induced axial histidine-flipping in myoglobin reconstituted with a cobalt tetradehydrocorrin as a methionine synthase model

Chem Commun (Camb). 2014 Oct 25;50(83):12560-3. doi: 10.1039/c4cc05448b.

Authors

Takashi Hayashi¹, Yoshitsugu Morita, Eiichi Mizohata, Koji Oohora, Jun Ohbayashi, Tsuyoshi Inoue, Yoshio Hisaeda

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan. thayashi@chem.eng.osaka-u.ac.jp.

PMID: 25197974
DOI: 10.1039/c4cc05448b

Abstract

A conjugate between apomyoglobin and cobalt tetradehydrocorrin was prepared to replicate the coordination behavior of cob(I)alamin in methionine synthase. X-ray crystallography reveals that the tetra-coordinated Co(I) species is formed through the cleavage of the axial Co-His93 ligation after the reduction of the penta-coordinated Co(II) cofactor in the heme pocket.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / chemistry*
Animals
Apoproteins / chemistry*
Cobalt / chemistry*
Crystallography, X-Ray
Escherichia coli / chemistry
Escherichia coli / enzymology*
Histidine / chemistry*
Horses
Models, Molecular
Myoglobin / chemistry*
Oxidation-Reduction
Vitamin B 12 / chemistry*

Substances

Apoproteins
Myoglobin
apomyoglobin
Cobalt
Histidine
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Vitamin B 12