Genome sequence of Ensifer arboris strain LMG 14919(T); a microsymbiont of the legume Prosopis chilensis growing in Kosti, Sudan

Wayne Reeve; Rui Tian; Lambert Bräu; Lynne Goodwin; Christine Munk; Chris Detter; Roxanne Tapia; Cliff Han; Konstantinos Liolios; Marcel Huntemann; Amrita Pati; Tanja Woyke; Konstantinos Mavrommatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Anne Willems

doi:10.4056/sigs.4828625

Genome sequence of Ensifer arboris strain LMG 14919(T); a microsymbiont of the legume Prosopis chilensis growing in Kosti, Sudan

Stand Genomic Sci. 2013 Dec 31;9(3):473-83. doi: 10.4056/sigs.4828625. eCollection 2014 Jun 15.

Authors

Wayne Reeve¹, Rui Tian¹, Lambert Bräu², Lynne Goodwin³, Christine Munk³, Chris Detter³, Roxanne Tapia³, Cliff Han³, Konstantinos Liolios⁴, Marcel Huntemann⁴, Amrita Pati⁴, Tanja Woyke⁴, Konstantinos Mavrommatis⁵, Victor Markowitz⁵, Natalia Ivanova⁴, Nikos Kyrpides⁴, Anne Willems⁶

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
² School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
³ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
⁴ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁵ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁶ Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Belgium.

Abstract

Ensifer arboris LMG 14919(T) is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of several species of legume trees. LMG 14919(T) was isolated in 1987 from a nodule recovered from the roots of the tree Prosopis chilensis growing in Kosti, Sudan. LMG 14919(T) is highly effective at fixing nitrogen with P. chilensis (Chilean mesquite) and Acacia senegal (gum Arabic tree or gum acacia). LMG 14919(T) does not nodulate the tree Leucena leucocephala, nor the herbaceous species Macroptilium atropurpureum, Trifolium pratense, Medicago sativa, Lotus corniculatus and Galega orientalis. Here we describe the features of E. arboris LMG 14919(T), together with genome sequence information and its annotation. The 6,850,303 bp high-quality-draft genome is arranged into 7 scaffolds of 12 contigs containing 6,461 protein-coding genes and 84 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.