[Construction and expression of the targeting super-antigen EGF-SEA fusion gene]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 May;28(9):638-40, 656.
[Article in Chinese]

Abstract

Objective: To construct expression vector for the SEA-EGF fusion gene.

Method: Clone the SEA gene and the EGF gene segment with PCR and RT-PCR independently, and connect this two genes by the bridge PCR. Insert the fusion gene EGF-SEA into the expression vector PET-44. Induced the secretion of the fusion protein SEA-EGF by the antileptic.

Result: The gene fragment encoding EGF and SEA mature peptide was successfully cloned. The fusion gene EGF-SEA was successfully constructed and was inserted into expression vector.

Conclusion: The new recombinant expression vector for fusion gene EGF-SEA is specific for head and neck cancer, laid the foundation for the further study of fusion protein SEA-EGF targeting immune therapy in head and neck tumors.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enterotoxins / genetics*
  • Epidermal Growth Factor / genetics*
  • Genetic Vectors*
  • Head and Neck Neoplasms / drug therapy
  • Humans
  • Molecular Targeted Therapy
  • Recombinant Fusion Proteins / biosynthesis*
  • Recombinant Fusion Proteins / genetics

Substances

  • Enterotoxins
  • Recombinant Fusion Proteins
  • enterotoxin A, Staphylococcal
  • Epidermal Growth Factor