A two-component pre-seeded dermal-epidermal scaffold

I P Monteiro; D Gabriel; B P Timko; M Hashimoto; S Karajanagi; R Tong; A P Marques; R L Reis; D S Kohane

doi:10.1016/j.actbio.2014.08.029

A two-component pre-seeded dermal-epidermal scaffold

Acta Biomater. 2014 Dec;10(12):4928-4938. doi: 10.1016/j.actbio.2014.08.029. Epub 2014 Sep 1.

Authors

I P Monteiro¹, D Gabriel², B P Timko², M Hashimoto², S Karajanagi³, R Tong², A P Marques⁴, R L Reis⁴, D S Kohane⁵

Affiliations

¹ Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory University of Minho, Braga/Guimarães, Portugal.
² Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
³ Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA.
⁴ 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory University of Minho, Braga/Guimarães, Portugal.
⁵ Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: daniel.kohane@childrens.harvard.edu.

Abstract

We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.

Keywords: Amine–aldehyde bonding; Bilayered; Hyaluronic acid; Skin defects.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biomimetic Materials
Cell Adhesion / physiology
Cell Proliferation / physiology
Cell Survival / physiology
Cells, Cultured
Equipment Design
Equipment Failure Analysis
Fibroblasts / cytology
Fibroblasts / physiology*
Fibroblasts / transplantation*
Humans
Keratinocytes / cytology
Keratinocytes / physiology*
Keratinocytes / transplantation*
Skin Transplantation / instrumentation*
Skin, Artificial*
Tissue Scaffolds*

Abstract

Publication types

MeSH terms

Grants and funding