Comparison of chromatographic conditions for analysis of selected psychotropic drugs in human serum

Abstract

Retention parameters of psychotropic drug standards were determined on different stationary phases: octadecyl silica, polar octadecyl silica, cyanopropyl silica and phenyl-hexyl silica using aqueous eluent systems containing acetonitrile, methanol or mixture of acetonitrile and methanol as organic modifiers; acetic buffer at pH 3.5 and diethylamine. The influence of stationary phases, kind of organic modifier and concentration of methanol and acetonitrile in mobile phases on retention, separation selectivity, peak symmetry and system efficiency was examined. These chromatographic parameters were significantly changed when analyses were performed on different stationary phases, when acetonitrile or methanol was used as organic modifier and when proportions of acetonitrile and methanol in eluent were different. The most efficient and selective systems were applied for quantification of the selected psychotropic drugs in fortified samples of human serum.