MiR-3960 binding sites with mRNA of human genes

Anatoly Ivashchenko; Olga Berillo; Anna Pyrkova; Raigul Niyazova; Shara Atambayeva

doi:10.6026/97320630010423

MiR-3960 binding sites with mRNA of human genes

Bioinformation. 2014 Jul 22;10(7):423-7. doi: 10.6026/97320630010423. eCollection 2014.

Authors

Anatoly Ivashchenko¹, Olga Berillo¹, Anna Pyrkova¹, Raigul Niyazova¹, Shara Atambayeva¹

Affiliation

¹ National Nanotechnology Laboratory, al-Farabi Kazakh National University, Almaty-050038, Kazakhstan.

Abstract

The importance of miRNA in cellular regulation is gaining momentum. Therefore, it is of interest to study miRNA in human genes. Hence, the humanmRNA sequences (12,175) were searched for miRNA binding sites and 2,563predicted sites were found. We observed that the miR-3960 has more than 1000mRNA binding sites with high affinity (with ΔG/ΔGm values greater than or equal to 90%) for 375genes. The miR-3960 has 565 binding sites in the 5'UTRs and 515 sites in theCDS of mRNAs. Nucleotide sequences of the binding sites in CDS encode for polyalanine orpolyproline. It is observed that miR-3960 has binding sites in 73 mRNAs of target genesencoded transcription factors. Thus, we document predictedproperties (polysites, sites in CDS) of uncharacterized miR-3960 binding sites. The studying of the miRNA properties is important for creation of diagnostic methods of cancer.

Keywords: Genes; MicroRNA; mRNA; miR-3960.