Next generation sequencing yields the complete mitochondrial genome of the Endangered Chilean silverside Basilichthys microlepidotus (Jenyns, 1841) (Teleostei, Atherinopsidae), validated with RNA-seq

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1505-6. doi: 10.3109/19401736.2014.953113. Epub 2014 Sep 4.

Abstract

The complete sequence of the mitochondrial genome for the Chilean silverside Basilichthys microlepidotus is reported for the first time. The entire mitochondrial genome was 16,544 bp in length (GenBank accession no. KM245937); gene composition and arrangement was conformed to that reported for most fishes and contained the typical structure of 2 rRNAs, 13 protein-coding genes, 22 tRNAs and a non-coding region. The assembled mitogenome was validated against sequences of COI and Control Region previously sequenced in our lab, functional genes from RNA-Seq data for the same species and the mitogenome of two other atherinopsid species available in Genbank.

Keywords: Freshwater fish; NGS; RNA-Seq; mitogenome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Endangered Species
  • Fishes / genetics*
  • Gene Order
  • Genome Size
  • Genome, Mitochondrial*
  • High-Throughput Nucleotide Sequencing / methods*
  • Mitochondria / genetics
  • Phylogeny
  • Sequence Analysis, DNA / methods
  • Sequence Analysis, RNA / methods*