Study of a monoclonal antibody specific to epithelial cells of mammalian thymuses

P Parvaz; C Auger; F Giraud; N Fabien; J R Faure; J C Monier

Study of a monoclonal antibody specific to epithelial cells of mammalian thymuses

J Clin Lab Immunol. 1989 Nov;30(3):135-9.

Authors

P Parvaz¹, C Auger, F Giraud, N Fabien, J R Faure, J C Monier

Affiliation

¹ Laboratoire d'Immunologie UFR Alexis Carrel, Lyon, France.

PMID: 2518390

Abstract

Hybridomas between spleen cells from BALB/c mice and Sp2/O-Ag14 mycloma cell line are performed by PEG fusion. An in vitro booster is realized with human thymic reticulo-epithelial cell cultures as antigen. A monoclonal antibody (3H9) directed against the thymic epithelium and stratified epithelia of mammals has been obtained and characterized.

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Epithelium / immunology
Female
Humans
Hybridomas / immunology
Immunoglobulin M / immunology
Immunoglobulin kappa-Chains / immunology
Mammals / immunology
Mice
Mice, Inbred BALB C / immunology
Organ Specificity
Species Specificity
Thymus Gland / immunology*

Substances

Antibodies, Monoclonal
Immunoglobulin M
Immunoglobulin kappa-Chains