Enterococcus faecium PBP5-S/R, the missing link between PBP5-S and PBP5-R

Ester Pietta; Maria Camila Montealegre; Jung Hyeob Roh; Pier Sandro Cocconcelli; Barbara E Murray

doi:10.1128/AAC.03648-14

Enterococcus faecium PBP5-S/R, the missing link between PBP5-S and PBP5-R

Antimicrob Agents Chemother. 2014 Nov;58(11):6978-81. doi: 10.1128/AAC.03648-14. Epub 2014 Sep 2.

Authors

Ester Pietta¹, Maria Camila Montealegre², Jung Hyeob Roh³, Pier Sandro Cocconcelli⁴, Barbara E Murray⁵

Affiliations

¹ Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Cremona, Italy.
² Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas, USA.
³ Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA.
⁴ Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Cremona, Italy.
⁵ Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas, USA Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas, USA bem.asst@uth.tmc.edu.

Abstract

During a study to investigate the evolution of ampicillin resistance in Enterococcus faecium, we observed that a number of E. faecium strains, mainly from the recently described subclade A2, showed PBP5 sequences in between PBP5-S and PBP5-R. These hybrid PBP5-S/R patterns reveal a progression of amino acid changes from the S form to the R form of this protein; however, these changes do not strictly correlate with changes in ampicillin MICs.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Amino Acid Sequence
Ampicillin / pharmacology
Ampicillin Resistance / genetics*
Anti-Bacterial Agents / pharmacology
Enterococcus faecium / drug effects*
Enterococcus faecium / enzymology*
Enterococcus faecium / genetics
Escherichia coli Proteins / genetics*
Genetic Variation
Microbial Sensitivity Tests
Molecular Sequence Data
Penicillin-Binding Proteins / genetics*
Protein Isoforms / genetics

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
PBP5 protein, E coli
Penicillin-Binding Proteins
Protein Isoforms
Ampicillin

Associated data

GENBANK/KJ742831
GENBANK/KJ742832
GENBANK/KJ742833
GENBANK/KJ742834
GENBANK/KJ742835

Grants and funding

R21 AI103260/AI/NIAID NIH HHS/United States