An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants

Annika Gillis; Jacques Mahillon

doi:10.1016/j.mimet.2014.08.012

An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants

J Microbiol Methods. 2014 Nov:106:101-103. doi: 10.1016/j.mimet.2014.08.012. Epub 2014 Aug 29.

Authors

Annika Gillis¹, Jacques Mahillon²

Affiliations

¹ Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Croix du Sud, Louvain-la-Neuve, Belgium. Electronic address: annika.gillis@uclouvain.be.
² Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Croix du Sud, Louvain-la-Neuve, Belgium.

PMID: 25179804
DOI: 10.1016/j.mimet.2014.08.012

Abstract

A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants.

Keywords: Bacillus thuringiensis; Phage; Phage-resistant mutants; Tectivirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus Phages / growth & development*
Bacillus thuringiensis / virology*
Microbiological Techniques / methods
Mutation*