Generation of osteoporosis in immune-compromised mice for stem cell therapy

Methods Mol Biol. 2014:1213:209-14. doi: 10.1007/978-1-4939-1453-1_17.

Abstract

To evaluate therapeutic efficacy and to investigate involved molecular mechanisms of cell-based therapy in osteoporosis, the generation of a clinically relevant model is critically important. Herein, we describe detailed methods in generation of an immune-deficient osteoporotic murine model, and application of human umbilical cord blood-derived stem cells to assess their therapeutic efficacy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / metabolism
  • Cell- and Tissue-Based Therapy*
  • Disease Models, Animal
  • Female
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Immunocompromised Host
  • Mice
  • Osteoporosis / etiology
  • Osteoporosis / therapy*
  • Stem Cell Transplantation*

Substances

  • Antigens, CD34