Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone-butanol-ethanol fermentation using an empirical model

Hafiza Shukor; Najeeb Kaid Nasser Al-Shorgani; Peyman Abdeshahian; Aidil Abdul Hamid; Nurina Anuar; Norliza Abd Rahman; Mohd Sahaid Kalil

doi:10.1016/j.biortech.2014.07.055

Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone-butanol-ethanol fermentation using an empirical model

Bioresour Technol. 2014 Oct:170:565-573. doi: 10.1016/j.biortech.2014.07.055. Epub 2014 Jul 21.

Authors

Hafiza Shukor¹, Najeeb Kaid Nasser Al-Shorgani², Peyman Abdeshahian³, Aidil Abdul Hamid⁴, Nurina Anuar⁵, Norliza Abd Rahman⁵, Mohd Sahaid Kalil⁶

Affiliations

¹ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia. Electronic address: ieza_72@yahoo.com.
² School of Biosciences and Biotechnology, Faculty of Sciences and Technology, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia. Electronic address: nag_nas2001@yahoo.com.
³ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia. Electronic address: peyman_137@yahoo.com.
⁴ School of Biosciences and Biotechnology, Faculty of Sciences and Technology, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia.
⁵ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia.
⁶ Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia. Electronic address: mskpmn@gmail.com.

PMID: 25171212
DOI: 10.1016/j.biortech.2014.07.055

Abstract

Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P<0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28°C and inoculum size of 15.9%. ABE fermentation was carried out under optimum conditions which 0.1g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70% in which 3.59g/L butanol was produced.

Keywords: Acid pretreatment; Butanol production; Clostridium saccharoperbutylacetonicum N1-4; Empirical model; Palm kernel cake.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetone / metabolism
Biofuels*
Butanols / metabolism*
Chromatography, High Pressure Liquid
Clostridium / metabolism*
Ethanol / metabolism
Fermentation
Lignin / analysis
Lignin / metabolism*
Models, Biological
Palm Oil
Plant Oils / chemistry*
Waste Products / analysis*

Substances

Biofuels
Butanols
Plant Oils
Waste Products
lignocellulose
Acetone
Ethanol
Palm Oil
Lignin