Fabrication of surface plasmon resonance sensor surface with control of the non-specific adsorption and affinity for the detection of 2,4,6-trinitrotoluene using an antifouling copolymer

Rui Yatabe; Takeshi Onodera; Kiyoshi Toko

doi:10.3389/fbioe.2014.00010

Fabrication of surface plasmon resonance sensor surface with control of the non-specific adsorption and affinity for the detection of 2,4,6-trinitrotoluene using an antifouling copolymer

Front Bioeng Biotechnol. 2014 Apr 29:2:10. doi: 10.3389/fbioe.2014.00010. eCollection 2014.

Authors

Rui Yatabe¹, Takeshi Onodera¹, Kiyoshi Toko²

Affiliations

¹ Research and Development Center for Taste and Odor Sensing, Kyushu University , Fukuoka , Japan.
² Graduate School of Information Science and Electrical Engineering, Kyushu University , Fukuoka , Japan.

Abstract

We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT). The hydrophilic polymer was made from mono-2-(methacryloyloxy)ethylsuccinate (MES) and 2-hydroxyethylmethacrylate (HEMA) by surface-initiated atom transfer radical polymerization. The detection of TNT was carried out by displacement assay with the SPR measurement. In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity. In the SPR measurement, non-specific adsorption should be controlled because SPR sensor cannot discriminate between specific and non-specific adsorption. Therefore, the affinity and non-specific adsorption were controlled by changing the ratio of HEMA to MES. A detection limit of 0.4 ng/ml (ppb) for TNT was achieved using a sensor surface with the lowest affinity without non-specific adsorption.

Keywords: displacement assay; immunosensor; non-specific adsorption; self-assembled monolayer; surface plasmon resonance; surface-initiated atom transfer radical polymerization; trinitrotoluene.