Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species

Barbara A Qurollo; Dana Riggins; Alaire Comyn; Merone T Zewde; Edward B Breitschwerdt

doi:10.1128/JCM.02340-14

Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species

J Clin Microbiol. 2014 Nov;52(11):4030-2. doi: 10.1128/JCM.02340-14. Epub 2014 Aug 20.

Authors

Barbara A Qurollo¹, Dana Riggins¹, Alaire Comyn¹, Merone T Zewde¹, Edward B Breitschwerdt²

Affiliations

¹ Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
² Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA ebbreits@ncsu.edou.

Abstract

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Dogs
Ehrlichia / genetics*
Ehrlichia / isolation & purification*
Ehrlichiosis / diagnosis*
Ehrlichiosis / microbiology
Ehrlichiosis / veterinary
Humans
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Superoxide Dismutase / genetics*

Substances

Bacterial Proteins
DNA, Bacterial
RNA, Ribosomal, 16S
SodB protein, Bacteria
Superoxide Dismutase

Associated data

GENBANK/KC778986