Characterization of a novel thermostable nuclease homolog (NucM) in a highly divergent Staphylococcus aureus clade

J Clin Microbiol. 2014 Nov;52(11):4036-8. doi: 10.1128/JCM.02327-14. Epub 2014 Aug 20.

Abstract

A thermostable nuclease homologue (NucM) in an animal-associated divergent clade of Staphylococcus aureus in sub-Saharan Africa has a highly divergent nucleotide sequence compared to those of the classical nuc1 and nuc2 genes of S. aureus. Its deduced amino acid sequences, tertiary structures, and nuclease activities, however, are similar.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa South of the Sahara
  • Amino Acid Sequence
  • Animals
  • Cluster Analysis
  • DNA / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Hydrolysis
  • Micrococcal Nuclease / genetics*
  • Micrococcal Nuclease / metabolism*
  • Models, Molecular
  • Molecular Sequence Data
  • Phylogeny
  • Protein Conformation
  • Sequence Analysis, DNA
  • Sequence Homology
  • Staphylococcal Infections / microbiology*
  • Staphylococcal Infections / veterinary
  • Staphylococcus aureus / enzymology*
  • Staphylococcus aureus / genetics

Substances

  • DNA, Bacterial
  • DNA
  • Micrococcal Nuclease

Associated data

  • GENBANK/KJ748637
  • GENBANK/KJ748638
  • GENBANK/KJ748639