The blood flow of esophageal substitute has been subjectively evaluated by surgeons. HyperEye Medical System (HEMS) allows for the visualization of the fluorescent image of indocyanine green (ICG) enhanced by near-infrared light among the surrounding vivid color images. We applied HEMS for evaluating the blood flow of right gastroepiploic artery and tissue blood flow of whole gastric tube during operation. Quantification of arterial blood flow and venous return has also been now developing for objective evaluation. We hope HEMS contributes to development of surgical results on various fields by establishing quantification of blood flow and clinical application.