The impact of interleukin 12B (1188A>C), interleukin 16 (-295T>C), and interleukin 18 (607C>A, 137G>C) gene polymorphisms on long-term renal transplant function and recipient outcomes

J Pawlus; A Sierocka; K Tejchman; Z Ziętek; M Romanowski; A Pawlik; J Sieńko; M Żukowski; K Ciechanowski; M Ostrowski; T Sulikowski

doi:10.1016/j.transproceed.2014.06.019

The impact of interleukin 12B (1188A>C), interleukin 16 (-295T>C), and interleukin 18 (607C>A, 137G>C) gene polymorphisms on long-term renal transplant function and recipient outcomes

Transplant Proc. 2014 Jul-Aug;46(6):2079-82. doi: 10.1016/j.transproceed.2014.06.019.

Authors

J Pawlus¹, A Sierocka¹, K Tejchman¹, Z Ziętek², M Romanowski¹, A Pawlik³, J Sieńko¹, M Żukowski⁴, K Ciechanowski⁵, M Ostrowski¹, T Sulikowski⁶

Affiliations

¹ Department of General Surgery and Transplantation, Pomeranian Medical University, Szczecin, Poland.
² Department of Anatomy, Pomeranian Medical University, Szczecin, Poland.
³ Department of Pharmacokinetics and drugs Monitoring, Pomeranian Medical University, Szczecin, Poland.
⁴ Department of Anesthesiology and Intensive Care, Pomeranian Medical University, Szczecin, Poland.
⁵ Department of Nephrology, Transplantology and Internal Diseases, Pomeranian Medical University, Szczecin, Poland.
⁶ Department of General Surgery and Transplantation, Pomeranian Medical University, Szczecin, Poland. Electronic address: sulikowskit@wp.pl.

PMID: 25131111
DOI: 10.1016/j.transproceed.2014.06.019

Abstract

Background: Inflammatory mediators play an important role in kidney graft outcome. The cytokine and chemokine gene polymorphisms are associated with variable production, activity, expression, or ligand-receptor affinity. Genetic variation in the DNA sequence of the interleukin 12B (IL12B), interleukin 16 (IL16), and interleukin 18 (IL18) genes may lead to altered cytokine production and activity. These variations can lead to changes in individual patient outcomes after kidney transplantation. It is known that polymorphisms of interleukins have an influence on inflammatory diseases, eg, Crohn's disease, diabetes, and asthma.

Aim: The aim of this study was to evaluate the correlation between IL12B, IL16, and IL18 gene polymorphisms with delayed graft function (DGF), acute rejection episodes (AR), and chronic rejection episodes (CR).

Materials and methods: A total of 267 (38.6% women, 61.4% men) recipients were included in the study. Cadaveric kidney transplantations were performed at the Department of General Surgery and Transplantation. Polymerase chain reaction was used to determine gene polymorphisms of IL12B (rs3212227), IL16 (4778889), and IL18 (rs1946518, rs187238) in 2 mL of serum. Statistical significance (P < .05) was analyzed by logit regression, ANOVA and odds ratio (OR) of χ(2) with Yates correction (95% confidence interval).

Results: Regression analysis revealed no significance between AR/DGF/CR and IL-2B, IL16, IL18rs1946518, and IL18-rs187238 (P > .05). The CR group, AA vs CC genotype of IL18 (rs1946518), had an OR = 2.35 (P = .04). AR and DGF groups had no significance in OR.

Conclusions: There was no statistical significance between IL12B, IL16, and IL18 (rs187238) gene polymorphisms and kidney graft outcome after transplantation. Presence of AA genotype (IL18-rs1946518) is connected with a 2.35 times higher risk of CR occurrence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cohort Studies
Delayed Graft Function / genetics*
Female
Genotype
Graft Rejection / genetics*
Humans
Interleukin-12 Subunit p40 / genetics*
Interleukin-16 / genetics*
Interleukin-18 / genetics*
Kidney Failure, Chronic / genetics
Kidney Failure, Chronic / surgery
Kidney Transplantation*
Male
Middle Aged
Polymorphism, Genetic / genetics*
Treatment Outcome

Substances

IL12B protein, human
IL18 protein, human
Interleukin-12 Subunit p40
Interleukin-16
Interleukin-18