Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides

Abstract

Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.

Keywords: antisense agents; cage compounds; developmental biology; gene expression; oligonucleotides.