Combination effects of baicalein with antibiotics against oral pathogens

Abstract

Background: Baicalein is one of the major flavonoids in Scutellaria baicalensis Georgi, which has long been used in Asia as herbal medicine. Several biological effects of baicalein, such as antiviral, anti-inflammatiom, anti-hepatotoxicity, and anti-tumour properties, have been reported.

Objective and design: In this study, the antibacterial activities of baicalein were investigated in combination with ampicillin and/or gentamicin against oral bacteria.

Results: Baicalein was determined with MIC and MBC values ranging from 80 to 320 and 160 to 640 μg/mL against oral bacteria. The range of MIC₅₀ and MIC₉₀ were 20-160 μg/mL and 80-320 μg/mL, respectively. The combination effects of baicalein with antibiotics were synergistic (FIC index <0.375-0.5 and FBCI <0.5) against oral bacteria. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 1-6 h of treatment with the MIC₅₀ of baicalein, regardless of whether it was administered alone or with ampicillin or gentamicin.

Conclusion: These results suggest that baicalein combined with other antibiotics may be microbiologically beneficial and not antagonistic.

Keywords: Baicalein; Fractional bactericidal concentration (FBC) index; Fractional inhibitory concentration (FIC) index; Minimum bactericidal concentrations (MBCs); Minimum inhibitory concentrations (MICs); Synergistic effect.