Analysis of isoamylase debranched starches with size exclusion chromatography utilizing PFG columns

Jelena Ciric; Albert J J Woortman; Katja Loos

doi:10.1016/j.carbpol.2014.05.093

Analysis of isoamylase debranched starches with size exclusion chromatography utilizing PFG columns

Carbohydr Polym. 2014 Nov 4:112:458-61. doi: 10.1016/j.carbpol.2014.05.093. Epub 2014 Jun 7.

Authors

Jelena Ciric¹, Albert J J Woortman², Katja Loos³

Affiliations

¹ Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands; Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands. Electronic address: jelenaciricnl@gmail.com.
² Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address: a.j.j.woortman@rug.nl.
³ Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands; Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands. Electronic address: k.u.loos@rug.nl.

PMID: 25129767
DOI: 10.1016/j.carbpol.2014.05.093

Abstract

Debranched starches were tested with a previously developed method for size exclusion chromatography (SEC) with multi detection utilizing different columns than usually used for the separation of starch in DMSO. A number of debranched starches were analyzed. This system allows good separation of amylose and amylopectin after debranching of starch, and provides quantitative information on the amylose content. Additionally molar mass versus hydrodynamic radii (Rh) distributions of various debranched starches show that the debranching was not 100% and that the differences in the structure of various starches can be followed.

Keywords: Chain-length distribution; Debranching; SEC; Starch.

Publication types

Research Support, Non-U.S. Gov't