Abstract
Dengue virus (DENV) is the most widespread arthropod-borne virus, and the number and severity of outbreaks has increased worldwide in recent decades. Dengue is caused by DENV-1, DENV- 2, DENV-3 and DENV-4 which are genetically distant. The species has been subdivided into genotypes based on phylogenetic studies. DENV-2, which was isolated from dengue fever patients during an outbreak in Piaui, Brazil in 2006/2007 was analyzed by sequencing the envelope (E) gene. The results indicated a high similarity among the isolated viruses, as well as to other DENV-2 from Brazil, Central America and South America. A phylogenetic and phylogeographic analysis based on DENV-2E gene sequences revealed that these viruses are grouped together with viruses of the American-Asian genotype in two distinct lineages. Our results demonstrate the co-circulation of two American-Asian genotype lineages in northeast Brazil. Moreover, we reveal that DENV-2 lineage 2 was detected in Piauí before it disseminated to other Brazilian states and South American countries, indicating the existence of a new dissemination route that has not been previously described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aedes
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Amino Acid Sequence
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Animals
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Antibodies, Viral / blood
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Base Sequence
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Brazil / epidemiology
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Caribbean Region / epidemiology
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Cell Line
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Child
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Child, Preschool
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DNA, Viral / genetics
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Dengue / epidemiology
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Dengue / transmission*
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Dengue / virology
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Dengue Virus / classification*
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Dengue Virus / genetics*
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Dengue Virus / isolation & purification
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Disease Outbreaks
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Female
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Genetic Variation
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Humans
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Immunoglobulin M / blood
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Infant
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Male
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Middle Aged
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Molecular Epidemiology
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Phylogeography
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Polymorphism, Single Nucleotide
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RNA, Viral / genetics
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Sequence Analysis, DNA
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Viral Envelope Proteins / genetics*
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Young Adult
Substances
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Antibodies, Viral
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DNA, Viral
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Immunoglobulin M
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RNA, Viral
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Viral Envelope Proteins
Grants and funding
This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), INCT and PRONEX-Dengue, Departamento de Ciência e Tecnologia do Ministério da Saúde (DECIT/MS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.